Is an alcoholic fixative fluid used for manual liquid-based cytology accurate to perform HPV tests?

Correspondence: Christian Garbar Department of Biopathology, Institute Jean Godinot, 1 avenue General Koenig BP 171, 51056 Reims Cedex, France Tel +33 3 2650 4266 Fax +33 3 2650 4274 Email [email protected] Abstract: In Europe, the alternative centrifuge method of liquid-based cytology is widely used in cervical screening. Turbitec (Labonord SAS, Templemars, France) is a centrifuge method of liquid-based cytology using an alcoholic fixative fluid, Easyfix (Labonord). It is now well accepted that the association of liquid-based cytology and human papillomavirus test is indissociable of cervical screening. The aim of this work was to demonstrate that Easyfix alcoholic fluid is reliable to perform Hybrid Capture 2 (QIAGEN SAS, Courtaboeuf, France). In this study, 75 patients with colposcopy for cervical lesions served as gold standard. A sample was collected, at random, for Easyfix fixative cytological fluid and for Digene Cervical Sampler (QIAGEN). The results of Hybrid Capture 2 (with relative light unit .1) showed no statistical difference, a positive Spearman’s correlation (r = 0.82, P , 0.0001), and a kappa value of 0.87 (excellent agreement) between the two fluids. It was concluded that Easyfix is accurate to use in human papillomavirus tests with Hybrid Capture 2.